The mechanism of eukaryotic translation initiation and principles of its regulation. Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling. Ingolia NT, Ghaemmaghami S, Newman JR, Weissman JS. Translational control in cancer etiology. Principles of translational control: an overview. Translational control of eukaryotic gene expression. Van Der Kelen K, Beyaert R, Inzé D, De Veylder L.
RIBOSOME PROFILING VIEWER SOFTWARE
Isoform-level Pipeline Ribo-seq Ribosome profiling Software Tool Visualization. We believe that RPiso is a useful tool for researchers to analyze and visualize their own ribosome profiling data at the isoform level. RPiso also provides a web-based viewer for interactively visualizing ribosome profiling data on the selected mRNA isoforms.
RIBOSOME PROFILING VIEWER INSTALL
RPiso is very easy to install and execute. In this study, we developed RPiso software tool ( ) to provide isoform-level ribosome profiling analyses. Compared to Ribomap (a widely used tool which provides isoform-level ribosome profiling analyses), our RPiso (1) estimates isoform abundance more accurately, (2) supports analyses on more species, and (3) provides a web-based viewer for interactively visualizing ribosome profiling data on the selected mRNA isoforms. Updated ribosome profiling and RNA-seq protocol Stephen Eichhorn, Bartel lab, JanuRibosome profiling and RNA-seq protocol Citation: Subtelny et al., Nature (2014) This protocol is an updated version of the protocol posted by Huili Guo (8/8/10), which detailed the approach she used for ribosome profiling and mRNA-seq in Guo et al. To allow other researchers to use our pipeline as well, here we implement our pipeline as an easy-to-use software tool called RPiso. To meet this need, previously we developed a pipeline to analyze 610 public human ribosome profiling data at the isoform level and constructed HRPDviewer database. Since different isoforms of a gene may produce different proteins with distinct biological functions, it is advantageous to analyze ribosome profiling data at the isoform level. However, almost all tools analyze ribosome profiling data at the gene level. Many ribosome profiling data processing tools have been developed. It can capture a snapshot of translation by deep sequencing of ribosome-protected mRNA fragments. Ribosome profiling (RP), also called ribo-seq, is a powerful experimental technique to study translational regulation. Dysregulation of translation results in abnormal cell physiology and leads to diseases. Mouse/Rat riboPOOL for Ribosome Profiling samples 12 rxn. These products include RiboLace Module 1, LaceSeq, PAGE Extraction Gel and UDI for 9 reactions. Translational regulation is one important aspect of gene expression regulation. All kits and reagants to perform ribosome profiling from cell lysis to sequencing and data analysis.